The utility of PacBio circular consensus sequencing for characterizing complex gene families in non-model organisms.

ABSTRACT:

Background

Molecular characterization of highly diverse gene families can be time consuming, expensive, and difficult, especially when considering the potential for relatively large numbers of paralogs and/or pseudogenes. Here we investigate the utility of Pacific Biosciences single molecule real-time (SMRT) circular consensus sequencing (CCS) as an alternative to traditional cloning and Sanger sequencing PCR amplicons for gene family characterization. We target vomeronasal gene receptors, one of the most diverse gene families in mammals, with the goal of better understanding intra-specific V1R diversity of the gray mouse lemur (Microcebus murinus). Our study compares intragenomic variation for two V1R subfamilies found in the mouse lemur. Specifically, we compare gene copy variati

SUBMITTER: Larsen PA 

PROVIDER: S-EPMC4152597 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature