Evaluation of candidate spermatogonial markers ID4 and GPR125 in testes of adult human cadaveric organ donors.

ABSTRACT: The optimal markers for human spermatogonial stem cells (SSCs) are not known. Among the genes recently linked to SSCs in mice and other animals are the basic helix-loop-helix transcription factor ID4 and the orphan G-protein-coupled receptor GPR125. While ID4 and GPR125 are considered putative markers for SSCs, they have not been evaluated for coexpression in human tissue. Furthermore, neither the size nor the character of the human spermatogonial populations that express ID4 and GPR125, respectively, are known. A major barrier to addressing these questions is the availability of healthy adult testis tissue from donors with no known reproductive health problems. To overcome this obstacle, we have employed healthy testicular tissue from a novel set of organ donors (n = 16; aged 17-68 years)

SUBMITTER: Sachs C 

PROVIDER: S-EPMC4153397 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature