Keratoconus is associated with increased copy number of mitochondrial DNA.

ABSTRACT:

Purpose

To investigate the possible association of oxidative stress with keratoconus (KC), we estimated the changes in relative mitochondrial DNA (mtDNA) content.

Methods

The study included 119 patients with KC and 208 controls matched for gender, ethnicity, and systemic disease status. We selected controls who were older than the patients since the mtDNA copy number tends to increase with age. The age mean (standard deviation) was 26.4(7.6) and 54.5(14.4) years for the patients and controls, respectively. The relative mtDNA copy number was estimated with the real-time quantitative PCR (qPCR) method using ND1 as the mtDNA gene and human globulin (HGB; also known as the cytoglobin gene, CYGB) as the reference single-copy nuclear gene.

Results

The mean relative mtDNA co

SUBMITTER: Abu-Amero KK 

PROVIDER: S-EPMC4153421 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature