The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing.

ABSTRACT:

Objective

An increasing number of studies that are using high-throughput molecular methods are rapidly extending our knowledge of gut microbial colonization in preterm infants whose immaturity and requirement for extensive treatment may result in altered colonization process. We aimed to describe the profile of gut microbiota in 50 extremely low birth weight (<1200 g) critically ill infants at three different time points during the first two months of life by using 16S rRNA gene specific sequencing.  

Patients and methods

Stool samples were collected at the age of one week, one month and two months. Bacterial community profiling was done using universal amplification of 16S rRNA gene and 454 pyrosequencing.

Results

The diversity of gut microbiota in preterm neonates i

SUBMITTER: Drell T 

PROVIDER: S-EPMC4153767 | biostudies-literature | 2014 May-Jun

REPOSITORIES: biostudies-literature