Association of global DNA methylation and global DNA hydroxymethylation with metals and other exposures in human blood DNA samples.

ABSTRACT:

Background

The association between human blood DNA global methylation and global hydroxymethylation has not been evaluated in population-based studies. No studies have evaluated environmental determinants of global DNA hydroxymethylation, including exposure to metals.

Objective

We evaluated the association between global DNA methylation and global DNA hydroxymethylation in 48 Strong Heart Study participants for which selected metals had been measured in urine at baseline and DNA was available from 1989-1991 (visit 1) and 1998-1999 (visit 3).

Methods

We measured the percentage of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in samples using capture and detection antibodies followed by colorimetric quantification. We explored the association of participan

SUBMITTER: Tellez-Plaza M 

PROVIDER: S-EPMC4154208 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature