Proteomic analysis of serum opsonins impacting biodistribution and cellular association of porous silicon microparticles.

ABSTRACT: Mass transport of drug delivery vehicles is guided by particle properties, such as size, shape, composition, and surface chemistry, as well as biomolecules and serum proteins that adsorb to the particle surface. In an attempt to identify serum proteins influencing cellular associations and biodistribution of intravascularly injected particles, we used two-dimensional gel electrophoresis and mass spectrometry to identify proteins eluted from the surface of cationic and anionic silicon microparticles. Cationic microparticles displayed a 25-fold greater abundance of Ig light variable chain, fibrinogen, and complement component 1 compared to their anionic counterparts. Anionic microparticles were found to accumulate in equal abundance in murine liver and spleen, whereas cationic microparticles

SUBMITTER: Serda RE 

PROVIDER: S-EPMC4154307 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature