Natural killer cell therapy and aerosol interleukin-2 for the treatment of osteosarcoma lung metastasis.

ABSTRACT:

Background

Survival of patients with osteosarcoma lung metastases has not improved in 20 years. We evaluated the efficacy of combining natural killer (NK) cells with aerosol interleukin-2 (IL-2) to achieve organ-specific NK cell migration and expansion in the metastatic organ, and to decrease toxicity associated with systemic IL-2.

Procedure

Five human osteosarcoma cell lines and 103 patient samples (47 primary and 56 metastatic) were analyzed for NKG2D ligand (NKG2DL) expression. Therapeutic efficacy of aerosol IL-2 + NK cells was evaluated in vivo compared with aerosol IL-2 alone and NK cells without aerosol IL-2.

Results

Osteosarcoma cell lines and patient samples expressed various levels of NKG2DL. NK-mediated killing was NKG2DL-dependent and correlated with expre

SUBMITTER: Guma SR 

PROVIDER: S-EPMC4154381 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature