Microfluidic platform for the quantitative analysis of leukocyte migration signatures.

ABSTRACT: Leukocyte migration into tissues is characteristic of inflammation. It is usually measured in vitro as the average displacement of populations of cells towards a chemokine gradient, not acknowledging other patterns of cell migration. Here, we designed and validated a microfluidic migration platform to simultaneously analyse four qualitative migration patterns: chemoattraction, -repulsion, -kinesis and -inhibition, using single-cell quantitative metrics of direction, speed, persistence and fraction of cells responding. We find that established chemokines, complement component 5a and IL-8 induce chemoattraction and repulsion in equal proportions, resulting in the dispersal of cells. These migration signatures are characterized by high persistence and speed and are independent of the chemokin

SUBMITTER: Boneschansker L 

PROVIDER: S-EPMC4155519 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature