MiR-506 suppresses proliferation of hepatoma cells through targeting YAP mRNA 3'UTR.

ABSTRACT:

Aim

MiR-506 is a miRNA involved in carcinogenesis of several kinds of cancer. In this study, we explored whether miR-506 played a critical role in hepatocellular carcinoma (HCC).

Methods

Twenty HCC and adjacent normal liver tissue samples were collected. Human hepatoma cell lines HepG2 and H7402 were used for in vitro studies. The expression of miR-506 and transcriptional co-activator YAP was examined using qRT-PCR. Western blot analysis was used to measure the expression of YAP and its target genes. Luciferase reporter gene assay was used to identify YAP as a target gene of miR-506. MTT and EdU assays were carried out for functional analysis.

Results

The expression of miR-506 was significantly lower in HCC than in adjacent normal liver tissues. Bioinformatics analysi

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4155531 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature