Matrix metalloproteinase 9 opposes diet-induced muscle insulin resistance in mice.

ABSTRACT:

Aims/hypothesis

Increased extracellular matrix (ECM) collagen is a characteristic of muscle insulin resistance. Matrix metalloproteinase (MMP) 9 is a primary enzyme that degrades collagen IV (ColIV). As a component of the basement membrane, ColIV plays a key role in ECM remodelling. We tested the hypotheses that genetic deletion of MMP9 in mice increases muscle ColIV, induces insulin resistance in lean mice and worsens diet-induced muscle insulin resistance.

Methods

Wild-type (Mmp9(+/+)) and Mmp9-null (Mmp9(-/-)) mice were chow or high-fat (HF) fed for 16 weeks. Insulin action was measured by the hyperinsulinaemic-euglycaemic clamp in conscious weight-matched surgically catheterised mice.

Results

Mmp9(-/-) and HF feeding independently increased muscle ColIV. ColIV in

SUBMITTER: Kang L 

PROVIDER: S-EPMC4155606 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature