Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6.

ABSTRACT: Human NEK7 is a regulator of cell division and plays an important role in growth and survival of mammalian cells. Human NEK6 and NEK7 are closely related, consisting of a conserved C-terminal catalytic domain and a nonconserved and disordered N-terminal regulatory domain, crucial to mediate the interactions with their respective proteins. Here, in order to better understand NEK7 cellular functions, we characterize the NEK7 interactome by two screening approaches: one using a yeast two-hybrid system and the other based on immunoprecipitation followed by mass spectrometry analysis. These approaches led to the identification of 61 NEK7 interactors that contribute to a variety of biological processes, including cell division. Combining additional interaction and phosphorylation assays from yea

SUBMITTER: de Souza EE 

PROVIDER: S-EPMC4156247 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature