The effect of freeze-thaw cycles on gene expression levels in lymphoblastoid cell lines.

ABSTRACT: Epstein-Barr virus (EBV) transformed lymphoblastoid cell lines (LCLs) are a widely used renewable resource for functional genomic studies in humans. The ability to accumulate multidimensional data pertaining to the same individual cell lines, from complete genomic sequences to detailed gene regulatory profiles, further enhances the utility of LCLs as a model system. However, the extent to which LCLs are a faithful model system is relatively unknown. We have previously shown that gene expression profiles of newly established LCLs maintain a strong individual component. Here, we extend our study to investigate the effect of freeze-thaw cycles on gene expression patterns in mature LCLs, especially in the context of inter-individual variation in gene expression. We report a profound difference

SUBMITTER: Calıskan M 

PROVIDER: S-EPMC4156430 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature