Global discovery and characterization of small non-coding RNAs in marine microalgae.

ABSTRACT:

Background

Marine phytoplankton are responsible for 50% of the CO2 that is fixed annually worldwide and contribute massively to other biogeochemical cycles in the oceans. Diatoms and coccolithophores play a significant role as the base of the marine food web and they sequester carbon due to their ability to form blooms and to biomineralise. To discover the presence and regulation of short non-coding RNAs (sRNAs) in these two important phytoplankton groups, we sequenced short RNA transcriptomes of two diatom species (Thalassiosira pseudonana, Fragilariopsis cylindrus) and validated them by Northern blots along with the coccolithophore Emiliania huxleyi.

Results

Despite an exhaustive search, we did not find canonical miRNAs in diatoms. The most prominent classes of sRNAs in di

SUBMITTER: Lopez-Gomollon S 

PROVIDER: S-EPMC4156623 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature