Frequent alteration of the protein synthesis of enzymes for glucose metabolism in hepatocellular carcinomas.

ABSTRACT:

Background

Cancer cells show enhanced glycolysis and inhibition of oxidative phosphorylation, even in the presence of sufficient oxygen (aerobic glycolysis). Glycolysis is much less efficient for energy production than oxidative phosphorylation, and the reason why cancer cells selectively use glycolysis remains unclear.

Methods

Biospecimens were collected from 45 hepatocellular carcinoma patients. Protein samples were prepared through subcellular localization or whole-cell lysis. Protein synthesis was measured by SDS-PAGE and immunoblotting. mRNA transcription was measured using quantitative RT-PCR. Statistical correlation among immunoblotting data and clinicolaboratory factors were analyzed using SPSS.

Results

Enzymes for oxidative phosphorylation (SDHA and SDHB) wer

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC4156784 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature