Study of single nucleotide polymorphism (rs28368082) in SPO11 gene and its association with male infertility.

ABSTRACT:

Purpose

The present study is a case-control analysis of a SNP (rs28368082) in exon 7 of the SPO11 gene and its possible association with male infertility in three provinces of Iran. We also searched for genetic differences among populations.

Methods

Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, we genotyped 113 infertile men and 50 fertile controls. Then, samples consisting SNP, as determined by PCR-RFLP, were genotyped by sequencing. The differences in genotype distributions between cases and fertile controls were examined using Chi-squared analysis. The genetic difference between individuals with mutated nucleotide was investigated by phylogenetic trees. Genetic difference among populations (provinces) was analyzed through AN

SUBMITTER: Ghalkhani E 

PROVIDER: S-EPMC4156955 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature