Roles of adherent myogenic cells and dynamic culture in engineered muscle function and maintenance of satellite cells.

ABSTRACT: Highly functional engineered skeletal muscle constructs could serve as physiological models of muscle function and regeneration and have utility in therapeutic replacement of damaged or diseased muscle tissue. In this study, we examined the roles of different myogenic cell fractions and culturing conditions in the generation of highly functional engineered muscle. Fibrin-based muscle bundles were fabricated using either freshly-isolated myogenic cells or their adherent fraction pre-cultured for 36 h. Muscle bundles made of these cells were cultured in both static and dynamic conditions and systematically characterized with respect to early myogenic events and contractile function. Following 2 weeks of culture, we observed both individual and synergistic benefits of using the adherent cell

SUBMITTER: Juhas M 

PROVIDER: S-EPMC4157105 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature