Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae.

ABSTRACT: The type VI secretion system (T6SS) is a macromolecular complex that is conserved in Gram-negative bacteria. The T6SS secretes effector proteins into recipient cells in a contact-dependent manner in order to accomplish cooperative and competitive interactions with the cells. Although the composition and mechanism of the T6SS have been intensively investigated across many Gram-negative bacteria, to date structural information on T6SS components from the important pathogen Vibrio cholerae has been rare. Here, the cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the cytoplasmic domain of TssL, an inner membrane protein of the T6SS, from V. cholerae are reported. Diffraction data were collected to 1.5 Å resolution using synchrotron radiation. The crysta

SUBMITTER: Jeong JH 

PROVIDER: S-EPMC4157432 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature