Different and overlapping functions of Arabidopsis LHT6 and AAP1 transporters in root amino acid uptake.

ABSTRACT: Plants acquire nitrogen in the form of amino acids from the soil, and transport proteins located in the plasma membrane of root cells are required for this process. It was found that the Arabidopsis lysine-histidine-like transporter LHT6 is expressed in root cells important for amino acid uptake, including the epidermis, root hairs, and cortex. Transport studies with lht6 mutants using high levels of amino acids demonstrated that LHT6 is in fact involved in amino acid uptake. To determine if LHT6 plays a role in nitrogen acquisition at soil amino acid concentrations, growth and uptake studies were performed with low levels of toxic amino acid analogues and radiolabelled amino acids, respectively. In addition, mutants of AAP1, another root amino acid transporter, and lht6/aap1 double mutant

SUBMITTER: Perchlik M 

PROVIDER: S-EPMC4157705 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature