Genome-wide binding and transcriptome analysis of human farnesoid X receptor in primary human hepatocytes.

ABSTRACT:

Background & aims

Farnesoid X receptor (FXR, NR1H4) is a ligand-activated transcription factor, belonging to the nuclear receptor superfamily. FXR is highly expressed in the liver and is essential in regulating bile acid homeostasis. FXR deficiency is implicated in numerous liver diseases and mice with modulation of FXR have been used as animal models to study liver physiology and pathology. We have reported genome-wide binding of FXR in mice by chromatin immunoprecipitation - deep sequencing (ChIP-seq), with results indicating that FXR may be involved in regulating diverse pathways in liver. However, limited information exists for the functions of human FXR and the suitability of using murine models to study human FXR functions.

Methods

In the current study, we performed Ch

SUBMITTER: Zhan L 

PROVIDER: S-EPMC4157742 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature