Genome-wide comparison of microRNAs and their targeted transcripts among leaf, flower and fruit of sweet orange.

ABSTRACT:

Background

In plants, microRNAs (miRNAs) regulate gene expression mainly at the post-transcriptional level. Previous studies have demonstrated that miRNA-mediated gene silencing pathways play vital roles in plant development. Here, we used a high-throughput sequencing approach to characterize the miRNAs and their targeted transcripts in the leaf, flower and fruit of sweet orange.

Results

A total of 183 known miRNAs and 38 novel miRNAs were identified. An in-house script was used to identify all potential secondary siRNAs derived from miRNA-targeted transcripts using sRNA and degradome sequencing data. Genome mapping revealed that these miRNAs were evenly distributed across the genome with several small clusters, and 69 pre-miRNAs were co-localized with simple sequence repeat

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4158063 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature