Dew inspired breathing-based detection of genetic point mutation visualized by naked eye.

ABSTRACT: A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions.

SUBMITTER: Xie L 

PROVIDER: S-EPMC4158330 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature