Mitochondrial metabolism directs stemness and differentiation in P19 embryonal carcinoma stem cells.

ABSTRACT: The relationship between mitochondrial metabolism and cell viability and differentiation in stem cells (SCs) remains poorly understood. In the present study, we compared mitochondrial physiology and metabolism between P19SCs before/after differentiation and present a unique fingerprint of the association between mitochondrial activity, cell differentiation and stemness. In comparison with their differentiated counterparts, pluripotency of P19SCs was correlated with a strong glycolytic profile and decreased mitochondrial biogenesis and complexity: round, low-polarized and inactive mitochondria with a closed permeability transition pore. This decreased mitochondrial capacity increased their resistance against dichloroacetate. Thus, stimulation of mitochondrial function by growing P19SCs in g

SUBMITTER: Vega-Naredo I 

PROVIDER: S-EPMC4158682 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature