Interactive transcriptome analysis of malaria patients and infecting Plasmodium falciparum.

ABSTRACT: To understand the molecular mechanisms of parasitism in vivo, it is essential to elucidate how the transcriptomes of the human hosts and the infecting parasites affect one another. Here we report the RNA-seq analysis of 116 Indonesian patients infected with the malaria parasite Plasmodium falciparum (Pf). We extracted RNAs from their peripheral blood as a mixture of host and parasite transcripts and mapped the RNA-seq tags to the human and Pf reference genomes to separate the respective tags. We were thus able to simultaneously analyze expression patterns in both humans and parasites. We identified human and parasite genes and pathways that correlated with various clinical data, which may serve as primary targets for drug developments. Of particular importance, we revealed characteristic e

SUBMITTER: Yamagishi J 

PROVIDER: S-EPMC4158759 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature