Microbiota modulate transcription in the intestinal epithelium without remodeling the accessible chromatin landscape.

ABSTRACT: Microbiota regulate intestinal physiology by modifying host gene expression along the length of the intestine, but the underlying regulatory mechanisms remain unresolved. Transcriptional specificity occurs through interactions between transcription factors (TFs) and cis-regulatory regions (CRRs) characterized by nucleosome-depleted accessible chromatin. We profiled transcriptome and accessible chromatin landscapes in intestinal epithelial cells (IECs) from mice reared in the presence or absence of microbiota. We show that regional differences in gene transcription along the intestinal tract were accompanied by major alterations in chromatin accessibility. Surprisingly, we discovered that microbiota modify host gene transcription in IECs without significantly impacting the accessible chroma

SUBMITTER: Camp JG 

PROVIDER: S-EPMC4158762 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature