Selective enrichment of damaged DNA molecules for ancient genome sequencing.

ABSTRACT: Contamination by present-day human and microbial DNA is one of the major hindrances for large-scale genomic studies using ancient biological material. We describe a new molecular method, U selection, which exploits one of the most distinctive features of ancient DNA--the presence of deoxyuracils--for selective enrichment of endogenous DNA against a complex background of contamination during DNA library preparation. By applying the method to Neanderthal DNA extracts that are heavily contaminated with present-day human DNA, we show that the fraction of useful sequence information increases ∼ 10-fold and that the resulting sequences are more efficiently depleted of human contamination than when using purely computational approaches. Furthermore, we show that U selection can lead to a four- to

SUBMITTER: Gansauge MT 

PROVIDER: S-EPMC4158764 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature