Insight into protein S-nitrosylation in Chlamydomonas reinhardtii.

ABSTRACT:

Aims

Protein S-nitrosylation, a post-translational modification (PTM) consisting of the covalent binding of nitric oxide (NO) to a cysteine thiol moiety, plays a major role in cell signaling and is recognized to be involved in numerous physiological processes and diseases in mammals. The importance of nitrosylation in photosynthetic eukaryotes has been less studied. The aim of this study was to expand our knowledge on protein nitrosylation by performing a large-scale proteomic analysis of proteins undergoing nitrosylation in vivo in Chlamydomonas reinhardtii cells under nitrosative stress.

Results

Using two complementary proteomic approaches, 492 nitrosylated proteins were identified. They participate in a wide range of biological processes and pathways, including photosynth

SUBMITTER: Morisse S 

PROVIDER: S-EPMC4158989 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature