Upregulation of 5-hydroxytryptamine receptor signaling in coronary arteries after organ culture.

ABSTRACT:

Background

5-Hydroxytryptamine (5-HT) is a powerful constrictor of coronary arteries and is considered to be involved in the pathophysiological mechanisms of coronary-artery spasm. However, the mechanism of enhancement of coronary-artery constriction to 5-HT during the development of coronary artery disease remains to be elucidated. Organ culture of intact blood-vessel segments has been suggested as a model for the phenotypic changes of smooth muscle cells in cardiovascular disease.

Methodology/principal findings

We wished to characterize 5-HT receptor-induced vasoconstriction and quantify expression of 5-HT receptor signaling in cultured rat coronary arteries. Cumulative application of 5-HT produced a concentration-dependent vasoconstriction in fresh and 24 h-cultured rat c

SUBMITTER: Deng CY 

PROVIDER: S-EPMC4159325 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature