Highly precise quantification of protein molecules per cell during stress and starvation responses in Bacillus subtilis.

ABSTRACT: Systems biology based on high quality absolute quantification data, which are mandatory for the simulation of biological processes, successively becomes important for life sciences. We provide protein concentrations on the level of molecules per cell for more than 700 cytosolic proteins of the Gram-positive model bacterium Bacillus subtilis during adaptation to changing growth conditions. As glucose starvation and heat stress are typical challenges in B. subtilis' natural environment and induce both, specific and general stress and starvation proteins, these conditions were selected as models for starvation and stress responses. Analyzing samples from numerous time points along the bacterial growth curve yielded reliable and physiologically relevant data suitable for modeling of cellular r

SUBMITTER: Maaβ S 

PROVIDER: S-EPMC4159648 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature