Identification of a novel neurotrophic factor from primary retinal Muller cells using stable isotope labeling by amino acids in cell culture (SILAC).

ABSTRACT: Retinal Müller glial cells (RMGs) have a primary role in maintaining the homeostasis of the retina. In pathological situations, RMGs execute protective and regenerative effects, but they can also contribute to neurodegeneration. It has recently been recognized that cultured primary RMGs secrete pro-survival factors for retinal neurons for up to 2 weeks in culture, but this ability is lost when RMGs are cultivated for longer durations. In our study, we investigated RMG supernatants for novel neuroprotective factors using a quantitative proteomic approach. Stable isotope labeling by amino acids in cell culture (SILAC) was used on primary porcine RMGs. Supernatants of RMGs cultivated for 2 weeks were compared with supernatants from cells that had already lost their protective capacity. Using

SUBMITTER: von Toerne C 

PROVIDER: S-EPMC4159655 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature