Tandem mass spectral libraries of peptides in digests of individual proteins: Human Serum Albumin (HSA).

ABSTRACT: This work presents a method for creating a mass spectral library containing tandem spectra of identifiable peptide ions in the tryptic digestion of a single protein. Human serum albumin (HSA(1)) was selected for this purpose owing to its ubiquity, high level of characterization and availability of digest data. The underlying experimental data consisted of ∼3000 one-dimensional LC-ESI-MS/MS runs with ion-trap fragmentation. In order to generate a wide range of peptides, studies covered a broad set of instrument and digestion conditions using multiple sources of HSA and trypsin. Computer methods were developed to enable the reliable identification and reference spectrum extraction of all peptide ions identifiable by current sequence search methods. This process made use of both MS2 (tandem)

SUBMITTER: Dong Q 

PROVIDER: S-EPMC4159660 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature