Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies.

ABSTRACT:

Aims

Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand-forelimb transplants performed at the University of Pittsburgh (PA, USA).

Materials & methods

The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute's Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation o

SUBMITTER: Donnenberg AD 

PROVIDER: S-EPMC4159957 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature