Identification of ENO1 as a potential sputum biomarker for early-stage lung cancer by shotgun proteomics.

ABSTRACT:

Background

Lung cancer is the leading cancer killer. Early detection will reduce the related deaths. The objective of this study was to identify potential biomarkers for early-stage lung cancer in sputum supernatant.

Materials and methods

Using shotgun proteomics, we detected changes in protein profiles that were associated with lung cancer by analyzing sputum supernatants from 6 patients with early-stage lung cancer and 5 cancer-free controls. Using western blotting, we validated the proteomic results in 22 lung cancer cases and 22 controls. Using enzyme-linked immunosorbent assay (ELISA), we evaluated the diagnostic performance of the biomarker candidates in an independent set of 35 cases and 36 controls.

Results

Proteomics identified 8 biomarker candidates for lung

SUBMITTER: Yu L 

PROVIDER: S-EPMC4160401 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature