Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers.

ABSTRACT:

Background

Class II myosins generate contractile forces in cells by polymerizing into bipolar filaments and pulling on anchored actin filaments. Nonmuscle myosin II (NMII) plays central roles during cell adhesion, migration, cytokinesis, and tissue morphogenesis. NMII is present in virtually all mammalian cell types as tissue-specific combinations of NMIIA, NMIIB, and NMIIC isoforms. It remains poorly understood how the highly dynamic NMII-actin contractile system begins to assemble at new cellular locations during cell migration and how incorporation of different NMII isoforms into this system is coordinated.

Results

Using platinum replica electron microscopy in combination with immunogold labeling, we demonstrate that individual activated (phosphorylated on the regulatory

SUBMITTER: Shutova MS 

PROVIDER: S-EPMC4160463 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature