Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

ABSTRACT: Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

SUBMITTER: Abrahamsson S 

PROVIDER: S-EPMC4161287 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature