Immunohistochemical toolkit for tracking and quantifying xenotransplanted human stem cells.

ABSTRACT:

Aim

Biomarker-based tracking of human stem cells xenotransplanted into animal models is crucial for studying their fate in the field of cell therapy or tumor xenografting.

Materials & methods

Using immunohistochemistry and in situ hybridization, we analyzed the expression of three human-specific biomarkers: Ku80, human mitochondria (hMito) and Alu.

Results

We showed that Ku80, hMito and Alu biomarkers are broadly expressed in human tissues with no or low cross-reactivity toward rat, mouse or pig tissues. In vitro, we demonstrated that their expression is stable over time and does not change along the differentiation of human-derived induced pluripotent stem cells or human glial-restricted precursors. We tracked in vivo these cell populations after transplantation in r

SUBMITTER: Allard J 

PROVIDER: S-EPMC4161450 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature