Modular extracellular sensor architecture for engineering mammalian cell-based devices.

ABSTRACT: Engineering mammalian cell-based devices that monitor and therapeutically modulate human physiology is a promising and emerging frontier in clinical synthetic biology. However, realizing this vision will require new technologies enabling engineered circuitry to sense and respond to physiologically relevant cues. No existing technology enables an engineered cell to sense exclusively extracellular ligands, including proteins and pathogens, without relying upon native cellular receptors or signal transduction pathways that may be subject to crosstalk with native cellular components. To address this need, we here report a technology we term a Modular Extracellular Sensor Architecture (MESA). This self-contained receptor and signal transduction platform is maximally orthogonal to native cellula

SUBMITTER: Daringer NM 

PROVIDER: S-EPMC4161666 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature