An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X protein.

ABSTRACT:

Background

H3N8 equine influenza virus (EIV) has caused disease outbreaks in horses across the world since its first isolation in 1963. However, unlike human, swine and avian influenza, there is relatively little sequence data available for this virus. The majority of published sequences are for the segment encoding haemagglutinin (HA), one of the two surface glycoproteins, making it difficult to study the evolution of the other gene segments and determine the level of reassortment occurring between sub-lineages.

Methods

To facilitate the generation of full genome sequences for EIV, we developed a simple, cost-effective and efficient method. M13-tagged primers were used to amplify short, overlapping RT-PCR products, which were then sequenced using Sanger dideoxynucleotide se

SUBMITTER: Rash A 

PROVIDER: S-EPMC4161859 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature