Pichia pastoris Aft1--a novel transcription factor, enhancing recombinant protein secretion.

ABSTRACT:

Background

The methylotrophic yeast Pichia pastoris is frequently used for the production of recombinant proteins. However, expression levels can vary depending on the target protein. Allowing for simultaneous regulation of many genes, which may elicit a desired phenotype like increased protein production, overexpression of transcription factors can be used to overcome expression bottlenecks. Here, we present a novel P. pastoris transcription factor currently annotated as Aft1, activator of ferrous transport.

Results

The promoter regions of key secretory P. pastoris genes were screened for fungal transcription factor binding sites, revealing Aft1 as an interesting candidate for improving secretion. Genome wide analysis of transcription factor binding sites suggested Aft1 to

SUBMITTER: Ruth C 

PROVIDER: S-EPMC4161868 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature