Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury.

ABSTRACT:

Background

Real-time quantitative PCR (qPCR) is a technique frequently used to measure changes in mRNA expression. To ensure validity of experimental findings, it is important to normalize the qPCR data to reference genes that are stable and unaffected by the experimental treatment to correct for variability among samples. Unlike in some models of neuropathic pain, reference genes for models of inflammatory injury have not been validated. This study examined four candidate reference genes in an effort to identify and validate optimal genes for normalization of transcriptional changes occurring in the dorsal horn of the spinal cord and the rostral ventromedial medulla (RVM) following intraplantar injection of complete Freund's adjuvant (CFA).

Results

The expression of hypoxan

SUBMITTER: Walder RY 

PROVIDER: S-EPMC4161874 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature