Alternative calibration strategies for the clinical laboratory: application to nortriptyline therapeutic drug monitoring.

ABSTRACT:

Background

The addition of a calibration curve with every run is both time-consuming and expensive for clinical mass spectrometry assays. We present alternative calibration strategies that eliminate the need for a calibration curve except as required by laboratory regulations.

Methods

We measured serum nortriptyline concentrations prospectively in 68 patients on 16 days over a 2-month period using a method employing calibration schemes that relied on the measurement of the response ratio (RR) corrected by the response factor (RF), i.e., a measurement of the RR for an equimolar mixture of the analyte and internal standard. Measurements were taken with contemporaneous RF (cRF) measurements as well as sporadic RF (sRF) measurements. The measurements with these alternative calib

SUBMITTER: Olson MT 

PROVIDER: S-EPMC4162085 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature