Impact of DNA repair pathways on the cytotoxicity of piperlongumine in chicken DT40 cell-lines.

ABSTRACT: Piperlongumine is a naturally-occurring small molecule with various biological activities. Recent studies demonstrate that piperlongumine selectively kills various types of transformed cells with minimal toxicity to non-transformed cells by inducing a high level of reactive oxygen species (ROS). ROS generates various types of DNA lesions, including base modifications and single strand breaks. In order to examine the contribution of ROS-induced DNA damage to the cytotoxicity by piperlongumine, various DNA repair-deficient chicken DT40 cell-lines with a single DNA repair gene deletion were tested for cellular sensitivity to piperlongumine. The results showed that cell lines defective in homologous recombination (HR) display hyper-sensitivity to piperlongumine, while other cell lines with a d

SUBMITTER: Okamoto S 

PROVIDER: S-EPMC4162141 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature