Multifunctional roles for the protein translocation machinery in RNA anchoring to the endoplasmic reticulum.

ABSTRACT: Signal sequence-encoding mRNAs undergo translation-dependent localization to the endoplasmic reticulum (ER) and at the ER are anchored via translation on Sec61-bound ribosomes. Recent investigations into the composition and membrane association characteristics of ER-associated mRNAs have, however, revealed both ribosome-dependent (indirect) and ribosome-independent (direct) modes of mRNA association with the ER. These findings raise important questions regarding our understanding of how mRNAs are selected, localized, and anchored to the ER. Using semi-intact tissue culture cells, we performed a polysome solubilization screen and identified conditions that distinguish polysomes engaged in the translation of distinct cohorts of mRNAs. To gain insight into the molecular basis of direct mRNA a

SUBMITTER: Jagannathan S 

PROVIDER: S-EPMC4162190 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature