Comparison of hepatocellular carcinoma miRNA expression profiling as evaluated by next generation sequencing and microarray.

ABSTRACT: MicroRNA (miRNA) expression profiling has proven useful in diagnosing and understanding the development and progression of several diseases. Microarray is the standard method for analyzing miRNA expression profiles; however, it has several disadvantages, including its limited detection of miRNAs. In recent years, advances in genome sequencing have led to the development of next-generation sequencing (NGS) technologies, which significantly advance genome sequencing speed and discovery. In this study, we compared the expression profiles obtained by next generation sequencing (NGS) with the profiles created using microarray to assess if NGS could produce a more accurate and complete miRNA profile. Total RNA from 14 hepatocellular carcinoma tumors (HCC) and 6 matched non-tumor control tissues

SUBMITTER: Murakami Y 

PROVIDER: S-EPMC4162537 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature