Preparation of samples for leaf architecture studies, a method for mounting cleared leaves.

ABSTRACT: Several recent waves of interest in leaf architecture have shown an expanding range of approaches and applications across a number of disciplines. Despite this increased interest, examination of existing archives of cleared and mounted leaves shows that current methods for mounting, in particular, yield unsatisfactory results and deterioration of samples over relatively short periods. Although techniques for clearing and staining leaves are numerous, published techniques for mounting leaves are scarce. • Here we present a complete protocol and recommendations for clearing, staining, and imaging leaves, and, most importantly, a method to permanently mount cleared leaves. • The mounting protocol is faster than other methods, inexpensive, and straightforward; moreover, it yields clear and per

SUBMITTER: Vasco A 

PROVIDER: S-EPMC4162665 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature