Hyb-Seq: Combining target enrichment and genome skimming for plant phylogenomics.

ABSTRACT:

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Premise of the study

Hyb-Seq, the combination of target enrichment and genome skimming, allows simultaneous data collection for low-copy nuclear genes and high-copy genomic targets for plant systematics and evolution studies. •

Methods and results

Genome and transcriptome assemblies for milkweed (Asclepias syriaca) were used to design enrichment probes for 3385 exons from 768 genes (>1.6 Mbp) followed by Illumina sequencing of enriched libraries. Hyb-Seq of 12 individuals (10 Asclepias species and two related genera) resulted in at least partial assembly of 92.6% of exons and 99.7% of genes and an average assembly length >2 Mbp. Importantly, complete plastomes and nuclear ribosomal DNA cistrons were assembled using off-target reads. Phylogenomic analyses d

SUBMITTER: Weitemier K 

PROVIDER: S-EPMC4162667 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature