Using small molecules as a new challenge to redirect metabolic pathway.

ABSTRACT: The presence of acetate in the bacterial medium leads to a reduction in the growth rate of cells and recombinant protein production. In this study, three compounds including propionic acid, lithium chloride and butyric acid were added to the medium which decreased acetate levels and enhanced recombinant protein production (alpha-synuclein). In fact, propionic acid and lithium chloride are both known as acetate kinase inhibitors. The results obtained in the case of butyric acid were similar to those of the two other compounds indicating that butyric acid may act through a mechanism similar to propionic acid and lithium chloride. Consequently, it was shown that the presence of each of these supplements (5-200 μM) increased recombinant alpha-synuclein production and cell density by approximat

SUBMITTER: Morshedi D 

PROVIDER: S-EPMC4162896 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature