Extended metAFLP approach in studies of tissue culture induced variation (TCIV) in triticale.

ABSTRACT: We present the development of the theoretical background of the metAFLP approach which allows for partition of complex variation into sequence changes, de novo methylation and demethylation of the regenerants derived via in vitro tissue culture methods in the case of triticale. It was demonstrated that, independent of whether andro- or embryogenesis was used for plant regeneration, the level of sequence changes identified between regenerants is about 10 %. Moreover, DNA demethylation prevails over de novo methylation of the regenerants compared to the donor plant. The metAFLP approach allows for the evaluation of numerous quantitative characteristics. For instance, one may quantify the number of sites unaffected by tissue culture approaches, global site DNA methylation etc. It is suggested

SUBMITTER: Machczynska J 

PROVIDER: S-EPMC4162973 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature