Genetic variation of the whole ICAM4 gene in Caucasians and African Americans.

ABSTRACT:

Background

Landsteiner-Wiener (LW) is the human blood group system Number 16, which comprises two antithetical antigens, LW(a) and LW(b) and the high-prevalence antigen LW(ab) . LW is encoded by the intracellular adhesion molecule 4 (ICAM4) gene. The ICAM4 protein is part of the Rhesus complex in the red cell membrane and is involved in cell-cell adhesion.

Study design and methods

We developed a method to sequence the whole 1.9-kb ICAM4 gene from genomic DNA in one amplicon. We determined the nucleotide sequence of Exons 1 to 3, the two introns, and 402-bp 5'-untranslated region (UTR) and 347-bp 3'-UTR in 97 Caucasian and 91 African American individuals.

Results

Seven variant ICAM4 alleles were found, distinct from the wild-type ICAM4 allele (GenBank KF712272), known

SUBMITTER: Srivastava K 

PROVIDER: S-EPMC4163062 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature