Real-time digitization of metabolomics patterns from a living system using mass spectrometry.

ABSTRACT: The real-time quantification of changes in intracellular metabolic activities has the potential to vastly improve upon traditional transcriptomics and metabolomics assays for the prediction of current and future cellular phenotypes. This is in part because intracellular processes reveal themselves as specific temporal patterns of variation in metabolite abundance that can be detected with existing signal processing algorithms. Although metabolite abundance levels can be quantified by mass spectrometry (MS), large-scale real-time monitoring of metabolite abundance has yet to be realized because of technological limitations for fast extraction of metabolites from cells and biological fluids. To address this issue, we have designed a microfluidic-based inline small molecule extraction system,

SUBMITTER: Heinemann J 

PROVIDER: S-EPMC4163111 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature