What protein charging (and supercharging) reveal about the mechanism of electrospray ionization.

ABSTRACT: Understanding the charging mechanism of electrospray ionization is central to overcoming shortcomings such as ion suppression or limited dynamic range, and explaining phenomena such as supercharging. Towards that end, we explore what accumulated observations reveal about the mechanism of electrospray. We introduce the idea of an intermediate region for electrospray ionization (and other ionization methods) to account for the facts that solution charge state distributions (CSDs) do not correlate with those observed by ESI-MS (the latter bear more charge) and that gas phase reactions can reduce, but not increase, the extent of charging. This region incorporates properties (e.g., basicities) intermediate between solution and gas phase. Assuming that droplet species polarize within the high el

SUBMITTER: Ogorzalek Loo RR 

PROVIDER: S-EPMC4163133 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature